Abstract
We have used the unlabeled antibody peroxidase-antiperoxidase (PAP) immunocytochemical method to demonstrate that antisera generated against rat neurophysin (anti-rNP) and arginine vasotocin (anti-AVT) react with material in the pars parvocellularis and pars magnocellularis of the nucleus preopticus (NPO) and in the neurohypophysis of sexually mature platyfish ( Xiphophorus maculatus). With anti-rNP and anti-AVT, long beaded axons were seen to extend between the NPO and the neurohypophysis. Neurophysin and arginine vasotocin are immunologically distinct entities as shown by control absorption procedures. The two appear to be elaborated and stored in similar locations and follow comparable routes of transport between the NPO and the pituitary gland.
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