Abstract
Two strains of Streptomyces, S. badius and S. virdosporus, when incubated with kraft lignin (Indulin ATR) produce water soluble acid precipitable compounds. We show by a combination of elemental analysis, amino acid analysis, and FTIR that these are complexes of lignin fragments strongly associated with proteins. The latter, which can be separated by solvent extraction, are added by the microorganisms themselves. The lignin fraction, which represents approximately 60% of the complex weight, consists of lower molecular weight, lower methoxyl content Indulin fragments. KeyWords: lignin, Indulin, lignin–protein complexes, Fourier transform infrared spectrophotometry, high performance size exclusion chromatography, Streptomyces.
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