The decreasing of NFκB level in gingival junctional epithelium of rat exposed to Porphyromonas gingivalis with application of 1% curcumin on gingival sulcus

Abstract

Background: Periodontal disease is a chronic, multi-factorial disease. Chronic periodontitis is one of the main causes of tooth loss. Chronic periodontitis is usually caused by Porphyromonas gingivalis (P. gingivalis). P. gingivalis can induce NF κ B activation resulting in the increasing of periodontal extracellular matrix degradation. Curcumin can inhibit NF κ B activation and reduce the severity of periodontal degradation. Purpose: This research was aimed to observe level of NF κ B in gingival junctional epithelium of rat exposed to Porphyromonas gingivalis with local administration of curcumin. Methods: Sixteen Wistar rat were divided into two groups. Group 1 (treatment) consisted of eight rat given 2 x 10 6 CFU/ml P. gingivalis and 1% curcumin. Meanwhile, group 2 (control) consisted of eight rat given 2 x 10 6 CFU/ml P. gingivalis only. GCF samples were collected from gingival sulcus. The samples were biochemically analyzed with ELISA method. Data were then analyzed statistically by using independent t-test ( α =0.05). Results: The examination of NF κ B level showed that there was significant difference between treatment group and control group (p<0.05). The level of NF κ B in the treatment group was significantly lower than the control group. Conclusion: It can be concluded that 1% curcumin application can reduce NF κ B level in gingival junctional epithelium of rat exposed to P. gingivalis.