Abstract
Encapsulation of cells in polymeric shells allowing for separation of biological material from produced factors may find application in the systems for biological processes regulation. Inadequate efficiency of existing therapeutic anticancer regiments and the rise of multi-drug resistant cancer cells have required investigations into novel anticancer strategies. Enhancement of apoptosis in tumors has been suggested as a new anticancer strategy. Pathogenic microorganisms may have the role as the source of agents for apoptotic therapy. Modified cells of Bacillus subtilis were encapsulated using layer-by-layer technique within polymeric shells for application in local anti-tumor therapy. The applied shells were modified with incorporated fullerene derivate to ensure the layers stability and integrity.The impact of modified nano-thin shells coated bacterial cells on human leukemia cells was evaluated in vitro. It was observed that coating with applied polyelectrolyte layers with incorporated fullerenol allowed for bacterial cells functioning during the culture period and the lethal impact on eukaryotic cells was observed. Applied membrane conformation allowing for functioning of encapsulated microorganisms may be recommended or coating shells for local anti-tumor treatment purposes.
Highlights
Separation of biologically active material from produced factors is receiving a great deal of interest regarding biotechnological usability
There was no impact of that difference on bacterial cells viability observed during short-time experiment in vitro
To visualize the polyelectrolyte layer attached to the bacterial cell, the fluorescently labeled polylysine was used
Summary
Separation of biologically active material from produced factors is receiving a great deal of interest regarding biotechnological usability. A system of biologically active material coated with polymeric shells insuring delivery of intact active factors and interspacing from host immunological system (in case of cell transplantation) may serve for biological processes regulation in biotechnological applications. In spite of efforts to control cancer, the mortality associated with this disease has been increasing in developed countries in the recent decade. Inadequate efficiency of existing therapeutic regiments and the rise of multi-drug resistant cancer cells are the main factors which require a broadening of investigations into novel anticancer strategies. Enhancement of apoptosis in tumors has been suggested recently as an optional anticancer strategy [1,2] and generation of apoptotic anticancer agents has been receiving attention. Pathogenic microorganisms may have the role as the source of agents for apoptotic therapy
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