The c-wave of the direct-current electroretinogram and the standing potential of the albino rabbit eye in response to repeated series of light stimuli with different interstimulus intervals.

Abstract

The direct-current electroretinogram and the standing potential of the eye of seven albino rabbits were recorded in response to repeated light stimuli, which were presented in four consecutive series. The intervals between the beginning of succeeding stimuli were 8 minutes, 4 minutes, 2 minutes and 70 seconds (series 1, 2, 3 and 4, respectively). Stimulus duration (10 seconds) and light intensity (6.8 x 10(4) lux) were constant during the experiments. The series lasted for 36-40 minutes, and each was preceded by 30 minutes of dark adaptation. During series 1, the end amplitudes of the a-, b- and c-waves were not significantly changed compared with the initial levels. During series 2, 3 and 4, the a-, b- and c-wave amplitudes were markedly reduced immediately after the first electroretinogram recording. The a- and b-waves then recovered to a limited extent, but the c-wave was more fully restored. A slight peak in the c-wave amplitude could be discerned 16-20 minutes after the start of recording. A decrease in the standing potential was seen 50-54 seconds after the start of light stimulation during all four series, and a peak occurred 12-16 minutes after the start of recording. The similarity in behavior between the c-wave and the standing potential suggests the operation of a pigment epithelial mechanism behind the more complete recovery of the c-wave amplitude. When electroretinogram amplitudes and standing potential levels are discussed, and when one experiment is compared with another one, it is important that adaptational and stimulus conditions, as well as time course, are well controlled and clearly specified.