Concentration-dependent effects of dopamine on the direct current electroretinogram of pigmented rabbits during prolonged intermittent recording.

Abstract

The direct-current (DC) electroretinogram (ERG) was studied in 24 pigmented rabbits. Four experiments were performed, each including six animals. One eye was injected intravitreally with 0.1 ml dopamine (DA) with an estimated concentration in the vitreous body of 0.0025 mM, 0.025 mM, 0.25 mM and 2.5 mM, respectively. The contralateral eye was injected with the same amount of saline. Following the injection the animals were dark adapted for 30 min and then exposed to repeated light stimuli of low intensity for almost 3 hours (series I: 1 stimulus per 3 min, 10 s duration, light intensity 6.8 x 10(2) lux). After another 30 min period of dark adaptation repeated light stimuli of high intensity were presented to the eyes (series II: 1 stimulus per 70 s, 10 s duration, light intensity 6.8 x 10(4) lux) for 33 min. In the control eyes, a slow increase with time of the a-, b- and c-wave amplitudes was observed during series I. During series II, the a- b- and c-wave amplitudes were markedly reduced between the first and the second light stimulus, but subsequently grew to a peak. The behavior of the ERG in the eyes injected with dopamine was not different from that observed in the control eyes at the lowest concentration of the drug. At higher concentrations the b- and c-wave amplitudes were reduced compared with the control eyes, and did not show the slow increase with time observed in these eyes during series I. Peak responses observed during series II in the control eyes was increasingly suppressed in the eyes treated with dopamine. Results of ERG recordings suggest that dopamine influences retinal adaptation in rabbits in a dose dependent manner.