Abstract
The gladiolus is one of the important cut flower with the utmost commercial value in India. Gladiolus has about 180 species and over 30,000 cultivars, of which over 20 are produced in India for production of flowers commercially. Botrytis grey mould (BGM) caused by the fungus Botrytis cinerea is one of the most destructive disease of the gladiolus. In a present study, a total 35 isolates of Botrytis were obtained from the gladiolus surveyed area. Colony morphological studies (texture, form, color, production and degree of sporulation) has been conducted using three types of solid media i.e. PDA (Potato Dextrose Agar), Czapeck’sDox Agar, Kritzmen and Netzer Agar medium. All B. cinerea strains gave maximum fungal mycelium growth on PDA (90.0 mm to 87.5mm) and CDA than K and N medium. Spores of Botrytis cinerea was also observed under Scanning Electron Microscope (SEM) and Phase Contrast Microscope (PCM) for conidial size and number of conidia. Maximum conidial size was recorded on PDA in comparison to CDA and KN media.
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