Abstract
Monoclonal antibody HI30 (CD45) was digested at 37℃for 22 hours with porcine pepsin in 0.1M citrate buffer, pH4.0. The productivity of F (ab') 2 was 52.4% (n =6) . Compared with HI30 the immune activity of F (ab') 2was almost the same. F (ab') 2-immunoliposomes were obtained by coupling the molecule of F (ab') 2 with drugliposomes by a sulphur-ester covalent bond. Compared with double sulphur bone method, this method was easier of operation and the F (ab') 2 to drug-liposomes coupling ratio was higher. Targeting tests in vitro proved that the F (ab') 2-immunoliposomes had good targeting function. Key words: F (ab') 2; immunoliposomes; covalent coupling; targeting test.
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