Abstract
The investigation of the steps in the biosynthesis of coumermycin have proved that δ-aminolevulinic acid is the precursor for the central 3-methylpyrrole-2,4-dicarboxylic acid of the coumermycins. It has also proved that proline is the precursor of the terminal pyrrole-2-carboxylic esters. In 1977, results from an investigation of the inhibition of DNA gyrase by nalidixic and oxolinic acids, were reported. It was found that the enzyme is inhibited by both of these acids, but that oxolinic acid is much more potent than nalidixic acid. The locus of action of oxolinic acid is entirely different from that of coumermycin A 1 . No cross-resistance was found between oxolinic acid inhibition and coumermycin A 1 inhibition of E. coli DNA gyrase. It was further discovered that in the absence of ATP and with a high concentration (6 mM) of magnesium ion, DNA gyrase will transform supercoiled DNA to the relaxed form, in exact opposition to what DNA gyrase usually does.
Published Version
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