The correlation between the soluble triggering receptor expressed on myeloid cells-1 and the janus kinase/signal transducer and activator of transcription (JAK/STAT) pathway in rats with abdominal sepsis

Abstract

Objective To investigate the correlation between the soluble triggering receptor expressed on myeloid cells-1(sTREM-1) and the janus kinase/signal transducer and activator of transcription (JAK/STAT) pathway in rats with abdominal sepsis. Methods Using a sepsis model produced by cecal ligation and puncture (CLP), Wistar rats were randomly(random number) divided into normal control group (n=6), sham-operated group (n=24, )CLP group (n=48) , AG490 (JAK2 inhibitor) treatment group (n=48) and rapamycin (RPM , STAT3 inhibitor) treatment group (n=48). At different intervals, the rats in each group were sacrificed, then the peripheral blood cells were harvested to detect the percent of CD4+ CD25+ Treg cells in CD4+ cells by flow cytometry. Meanwhile, mRNA of sTREM-1 from the tissue samples of the liver was also measured by real-time reverse transcription-polymerase chain reaction (RT-PCR). Results Compared with the normal control group and the sham-operated group, expression of sTREM-1 in the CLP group was significantly higher, and it showed a gradually increased tendency over time. At 6 and 24 hours, there were no significant differences in the expressions of sTREM-1 between the AG490 group(1.572±0.051, 2.063±0.025, respectively)and the CLP group (1.592±0.036, 2.082±0.021, respectively). But the expression of sTREM-1in the AG490 group (2.522±0.083, 3.153±0.021, respectively)was lower than those in the CLP group (2.592±0.055, 3.204±0.013, respectively)during 48 and 72 hours. In addition, there was no significant difference in the expression of sTREM-1 between the RPM group (1.581±0.017) and the CLP group (1.592±0.036) at 6 hours. And the expression of sTREM-1in the RPM group (1.486±0.019, 1.263±0.011, 1.115±0.022, respectively)was significantly lower than those in the CLP group (2.082±0.021, 2.592±0.055, 3.204±0.013, respectively)during 24 to 72 hours. Conclusions These data proved that blocking JAK/STAT pathway could inhibit the expression of sTREM-1 and decelarate the progress of inflammatory reaction in rats with abdominal sepsis. Key words: Sepsis; Soluble triggering receptor expressed on myeloid cells-1; JAK/STAT; AG490; Rapamycin; Cecal ligation and puncture