The correlation between single nucleotide polymorphisms of the thymic stromal lymphopoietin receptor and breast cancer in a cohort of female patients in Saudi Arabia.

Abdelhabib Semlali,Mahmoud Rouabhia,Bader O Almutairi,Mikhlid H Almutairi,Sultan N Alharbi,Abdulwahed F Alrefaei,Abdullah M Alamri

doi:10.1007/s11356-021-15242-1

Abstract

The current study aimed to examine thymic stromal lymphopoietin receptor (TSLPR) genetic variation and breast cancer (BC) susceptibility in women in Saudi Arabia. Therefore, 127 blood samples from female patients diagnosed with BC and 116 blood samples from healthy female controls were studied using a genotyping assay to determine the association between three TSLPR single nucleotide polymorphisms (SNPs)-P196L, X201W, and A238V-and the risk of BC progression. In addition, gene expression was evaluated in 20 matching BC and normal tissues using immunohistochemistry. TSLPR protein levels were higher among BC patients than those with matching normal breast tissue. In addition, TSLPR SNP P196L was found to have a significant protective effect on BC progression (OR = 0.4427), although only the T allele for TSLPR P196L had this protective effect against BC progression in participants who were younger than 48 years old. In contrast, no association was found between the T allele and risk of BC in participants who were older than 48 years old, and the CT and TT genotypes were significantly associated with BC risk protection in the older group. The effects of the TT genotype and the T allele were closely associated with a decreased risk of BC in participants with estrogen receptors (ER+) and without them (ER-). Overall, the findings revealed a significant correlation between SNPs in the TSLPR genes and BC progression among women in Saudi Arabia.

Highlights

Breast cancer (BC) is a main cause of morbidity and mortality among females around the world, especially in industrialized countries, and is the main cause of death among women in Saudi Arabia
thymic stromal lymphopoietin receptor (TSLPR) immunostaining revealed that the BC epitheliums in the tissues surrounding the malignant tumor had a much higher degree of positive staining than the normal breast tissues (Figure 1)
We examined whether genetic variants of TSLPR were correlated with the BC group and/or the control group

Summary

Introduction

Breast cancer (BC) is a main cause of morbidity and mortality among females around the world, especially in industrialized countries, and is the main cause of death among women in Saudi Arabia. It has been shown that a Th2 response largely dominated the cytotoxicity induced by CD8 T cells and T-helper 1 (Th1) response in many types of cancers (Loose and Van de Wiele, 2009, Pedroza-Gonzalez et al, 2011). Tumors with this type of phenotype are more aggressive than tumors in which Th1-like responses predominate (Aspord et al, 2007, De Monte et al, 2011)

Objectives

Methods

Results

Discussion

Conclusion