Abstract
Objective The expression and correlation between cannabinoid receptor type 2(CB2R) and autophagy-related protein microtubule-associated protein 1 light chain 3(LC3) in the hippocampal CA1 region of developing rats with status epilepticus(SE) were investigated. Methods SE model was established using lithium chloride-pilocarpine intraperitoneally in Sprague-Dawley(SD) rats and all the rats were randomly divided into four groups( control group and 3h, 24h, 3d groups after SE). The expressions of CB2R and LC3 in the hippocampal CA1 region at different times were observed using double-label immunofluorescence and Western blotting.Spearman correlational analysis was used to compare the relationship between the two factors. Results Immunofluorescence showed that the expression of CB2R was up-regulated dynamically and peaked at 24h and presented parabolic changes over time.The expression of LC3 changed in accordance with CB2R and even co-expressed with CB2R partly, especially on neurons.Western blotting results furtherly showed the similarity of the expression of CB2R and LC3, and finally Spearman correlational analysis presented the significant correlation between these two factors(r=0.7161, P<0.05). Conclusion Significant correlation exists between the expression of CB2R and LC3 in the hippocampal CA1 neurons of developing rats with SE, indicating the essential role of CB2R in autophagy regulation of hippocampal CA1 neurons. Key words: Status epilepticus; Hippocampus; Cannabinoid receptor type 2; Autophagy; Microtubule-associated protein 1 light chain 3
Published Version
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