The correlation between anti-human leukocyte antibody and anti-endothelial cell antibody in chronic rejection after renal transplantation

Abstract

Objective To explore the role of anti-endothelial cell antibody and anti-human leukocyte antigen(HLA)antibody in chronic rejection after renal transplantation. Methods Immunofluorescence assay was performed to detect anti- endothelial cell antibody, and enzyme linked immunosorbent assay(ELISA)was performed to determine HLA antibody in the sera of patients after renal transplantation.The levels of creatinine in sera of the patients were also determined. Result The average level of creatinine was(116.3±5.6)μmol/L in the patients with positive anti-HLA antibody.The average level of creatinine was(114.6±4.7)μmol/L in the patients with positive anti-endothelial cell antibody after renal transplantation.The median level of creatinine was 131.2 μmol/L in the patients with both positive antibodies, and the quartile was 13.6 μmol/L.The levels of creatinine were significantly different between these two groups in statistical(P=0.000). Conclusion Both anti-endothelial cell antibody and anti-HLA antibody could lead to deterioration of renal function in chronic rejection reaction of renal transplant.The renal dysfunction was more serious when both anti-endothelial cell antibody and anti-HLA antibody were generated during chronic renal allograft rejection. Key words: Renal transplant; Anti-human leukocyte antigen antibody; Anti-endothelial cell antibody; Chronic rejection reaction