The role of anti-endothelial cell antibodies in chronic rejection after renal transplantation

Abstract

Objective To explore the role of non-HLA antibodies in chronic renal allograft rejection.Methods Immunofluoreseence assay was performed to detect anti-endothelial cell antibodies in the serum samples of 252 subjects including 72 patients receiving dialysis before renal transplantation,100 patients after renal transplantation and 80 normal blood donors.Result Anti-endothelial cell antibodies were detected in 38.89％ (28/72) patients before renal transplantation.The strong positive rate accounted for 13.89％ (10/72),and the weak positive rate accounted for 25.00％ (18/72).Anti-endothelial cell antibodies were detected in 70.00％ (70/100) patients after transplantation,in which the strong positive rate accounted for 38.00％ (38/ 100),while the weak positive rate accounted for 32.00％ (32/100).Anti-endothelial cell antibodies were detected 30.00％ (24/80) in normal blood donors,in which the strong positive rate was 10.00％ (8/80) and the weak positive rate was 20.00％ (16/80).The levels of anti-endothelial cell antibodies showed no difference between the pre-transplant group and normal control group (t =1.0105,P =0.1566).The levels of anti-endothelial cell antibodies were significantly different between pre-transplant group and post-transplant group (t =4.3311,P =0.0000).The levels of anti-endothelial cell antibodies showed significant difference between post-transplant group and normal control group (t =5.5572,P =0.0000).Conclusion Anti-endothelial cell antibodies were one of the important factors in chronic renal allograft rejection. Key words: Renal transplantation; Anti-endothelial cell antibody; Non-HLA antibodies; Chronic rejection reaction