The coronatine-insensitive 1 Mutation Reveals the Hormonal Signaling Interaction between Abscisic Acid and Methyl Jasmonate in Arabidopsis Guard Cells. Specific Impairment of Ion Channel Activation and Second Messenger Production

Shintaro Munemasa,Kenji Oda,Yasuaki Shimoishi,Megumi Watanabe-Sugimoto,Yoshimasa Nakamura,Yoshiyuki Murata

doi:10.1104/pp.106.091298

Abstract

Methyl jasmonate (MeJA) elicits stomatal closing similar to abscisic acid (ABA), but whether the two compounds use similar or different signaling mechanisms in guard cells remains to be clarified. We investigated the effects of MeJA and ABA on second messenger production and ion channel activation in guard cells of wild-type Arabidopsis (Arabidopsis thaliana) and MeJA-insensitive coronatine-insensitive 1 (coi1) mutants. The coi1 mutation impaired MeJA-induced stomatal closing but not ABA-induced stomatal closing. MeJA as well as ABA induced production of reactive oxygen species (ROS) and nitric oxide (NO) in wild-type guard cells, whereas MeJA did not induce production of ROS and NO in coi1 guard cells. The experiments using an inhibitor and scavengers demonstrated that both ROS and NO are involved in MeJA-induced stomatal closing as well as ABA-induced stomatal closing. Not only ABA but also MeJA activated slow anion channels and Ca(2+) permeable cation channels in the plasma membrane of wild-type guard cell protoplasts. However, in coi1 guard cell protoplasts, MeJA did not elicit either slow anion currents or Ca(2+) permeable cation currents, but ABA activated both types of ion channels. Furthermore, to elucidate signaling interaction between ABA and MeJA in guard cells, we examined MeJA signaling in ABA-insensitive mutant ABA-insensitive 2 (abi2-1), whose ABA signal transduction cascade has some disruption downstream of ROS production and NO production. MeJA also did not induce stomatal closing but stimulated production of ROS and NO in abi2-1. These results suggest that MeJA triggers stomatal closing via a receptor distinct from the ABA receptor and that the coi1 mutation disrupts MeJA signaling upstream of the blanch point of ABA signaling and MeJA signaling in Arabidopsis guard cells.

Highlights

Methyl jasmonate (MeJA) elicits stomatal closing similar to abscisic acid (ABA), but whether the two compounds use similar or different signaling mechanisms in guard cells remains to be clarified
The similar time course of stomatal movements was observed in coi1 plants treated with ABA, but there was no change in stomatal aperture of coi1 plants treated with MeJA (Fig. 1D)
Our results indicated that the coi1 mutation impaired MeJA-induced stomatal closing but not ABA-induced stomatal closing

Summary

Introduction

Methyl jasmonate (MeJA) elicits stomatal closing similar to abscisic acid (ABA), but whether the two compounds use similar or different signaling mechanisms in guard cells remains to be clarified. ICa channels are activated by ABA and fungal elicitors only in the presence of cytosolic NAD(P)H (Murata et al, 2001; Klusener et al, 2002), and activation of ICa channels by ABA is abolished in the Arabidopsis ABA-insensitive mutants gca (Pei et al, 2000) and ABA-insensitive 2 (abi; Murata et al, 2001). Induced cytosolic NAD(P)H-dependent activation of ICa channels are impaired in the Arabidopsis double mutant of catalytic subunits of plasma membrane

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Conclusion