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Abstract
Eel (Monopterus albus) is an aquatic biota that have a high nutrient content such as fatty acids and cholesterol. The purpose of this research was to determine the chemical composition, fatty acid, cholesterol, and description of tissue in fresh dan boiled eel. The research was carried out in several steps include sampling, sample preparation, morphometric and yield calculations, boiling eel for 20 minutes at 100ºC. Analysis performed in fresh and boiled eel was proximate analysis, fatty acids, cholesterol, and observation of tissue structure. The highest content of saturated fatty acids (SFA) in fresh eel was palmitic acid 13.79%, the highest content of monounsaturated fatty acid (MUFA) was oleic acid 19.45%, the highest content of polyunsaturated fatty acid (PUFA) was linoleic acid 7.42%. The content of fatty acid and cholesterol in eel has changed overall due to the boiling process. The content of cholesterol were 60 mg/100 g (in fresh eel) and 56.32 mg/100 g (in boiled sample). The structure of eel’s tissue changed due the boiling process.<br />Keywords: cholesterol, eel (Monopterus albus), fatty acid, tissue structure
Highlights
Eel (Monopterus albus) is an aquatic biota that have a high nutrient content such as fatty acids and cholesterol
The structure of eel's tissue changed due the boiling process
Effect of different cooking methods on the oxidation, proximate and fatty acid composition of silver catfish (Rhamdia quelen) fillets
Summary
Bahan utama yang digunakan dalam penelitian ini adalah belut (M. albus) yang didapat dari Pasar Anyar, Bogor, Jawa Barat. Bahan yang digunakan meliputi NaOH 0,5 N dalam metanol, BF3 20%, larutan NaCl jenuh, n-heksana, Na2SO4 anhidrat, etanol, petroleum benzen, alkohol, H2SO4 pekat, FeCl3.6H2O, K2SO4, parafin, kain kasa, pewarna hematoksilin-eosin, alkohol, xilol dan larutan BNF. Alat yang digunakan meliputi homogenizer, evaporator, Erlenmeyer, corong pisah, botol vial, vortex, botol film, mikrotom, waterbath, mortar, syringe 10 μL, penangas air, pipet mikro, tabung bertutup teflon, neraca analitik, kromatografi gas (Shimadzu 2010 Plus), mikroskop (Olympus CX 41), spektrofotometer (Shimadzu UV-1700)
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