The compost metagenome as a source of T4 bacteriophage pyrimidine dimer glycosylase homologues

Alexandra N Karmanova,Andrei A Zimin,Yinhua Lü

doi:10.1051/e3sconf/202126504009

Abstract

Compost is a promising source of thermotolerant enzymes for their application in biotechnology. Homologues of bacteriophage T4 DNA glycosylase can find their application in pharmaceuticals and perfumery. Five homologues of glycosylase of pyrimidine dimers of bacteriophage T4, a product of the denV gene, were found by comparing using the DELTA-BLAST algorithm with the compost metagenome proteins. Phylogenetic analysis of the found sequences of enzyme homologues was carried out using the Maximum Likelihood algorithm in the MegaX software package. Thus, an interesting spectrum of promising proteins, homologues of the repair enzyme, DNA glycosylase of pyrimidine dimers of bacteriophage T4, was found. After structural modeling, they can be tested for their thermal stability and tested as a basis for therapeutic and prophylactic drugs.

Highlights

Many enterprises and farms, whose activities involve the presence of biowaste, use various methods of composting to process certain types of organic matter
Composting implies a process of aerobic decomposition of waste as a result of the vital activity of microorganisms to water, carbon dioxide, heat and the final product - compost [1]
The latter, as a rule, is subsequently used as an organic fertilizer for the soil. It can act as a source of microorganisms with enzymes, which can be further used in various industries

Summary

Introduction

Many enterprises and farms, whose activities involve the presence of biowaste, use various methods of composting to process certain types of organic matter. This is justified both by the ecological purity and safety of the method, and by its economic value. Composting implies a process of aerobic decomposition of waste as a result of the vital activity of microorganisms to water, carbon dioxide, heat and the final product - compost [1]. The latter, as a rule, is subsequently used as an organic fertilizer for the soil. The most promising is the selection of biochemical catalysts for the production of biofuels, the processing of cellulose, plastic and other hard-to-decompose substances, as well as the search for biomolecules that can form the basis of pharmaceuticals

Objectives

Methods

Results