Abstract
AbstractAn occluding substance (gum) was observed in the vessels ofPhellodendron amurenseRupr. and analysed by spectroscopic and chemical methods. Following safranin-alcian blue staining, the gum in sapwood (sW) turned to blue and in heartwood (hW) to red. The gum was studiedin situby UV and Raman microscopies, time-of-flight secondary ion mass spectrometry (TOF-SIMS). The gum was isolated by laser microdissection (LMD) and it was alkali hydrolysed and the degradation products were analysed by GC-MS. The staining experiments, and the UV and Raman microscopies indicated that the major component of the sW gum is constituted of polysaccharides, while in the hW gum the aromatic character is dominating. TOF-SIMS measurements were interpreted as showing the aromatic substances in the hW gum did not contain lignin. The GC-MS analysis revealed the presence of vanillic acid in the degradation products of hW gum.
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