Abstract
Aim. To study the qualitative composition and the quantitative content of the essential oil components from Laurus nobilis L. unripe fruits.Results and discussion. In the essential oil from Laurus nobilis L. unripe fruits 31 compounds were determined; among them 28 substances were identified. A high content was characteristic for spatulenol (1947.1 mg/kg) and betulenol (925.3 mg/kg).Experimental part. The raw material for obtaining the essential oil (unripe fruits) of Laurus nobilis L. was harvested in November 2017 in Alushta and the village of Rybalskoe, Crimea. The component composition of the essential oil of the unripe fruits from Laurus nobilis L. was studied and the constituents of the essential oil were identified by chromato-mass spectrometry using an Agilent Technology 6890N chromatograph. The component composition of the essential oil was revealed by comparing the results obtained with data from the NIST 02 mass spectrum library (more than 174.000 substances).Conclusions. The component composition of the essential oil from Laurus nobilis L. unripe fruits harvested in Ukraine has been studied. Taking into account the set of the biologically active compounds found in the essential oil of Laurus nobilis L. it can be argued that further pharmacognostic studies of fruits of this plant as the medicinal raw material with the predicted pharmacological activity (e.g., antimicrobial and skin cleansing) are promising.
Highlights
The component composition of the essential oil from Laurus nobilis L. unripe fruits harvested in Ukraine Aim
The raw material for obtaining the essential oil of Laurus nobilis L. was harvested in November 2017 in Alushta and the village of Rybalskoe, Crimea
The component composition of the essential oil of the unripe fruits from Laurus nobilis L. was studied and the constituents of the essential oil were identified by chromato-mass spectrometry using an Agilent Technology 6890N chromatograph
Summary
Об’єктом дослідження є ефірна олія нестиглих плодів сировини лавру благородного українського зростання. Одержання ефірної олії лавру благородного Наважку (60,0 г) подрібненої сировини переносили в колбу об’ємом 1 л, додавали 300 мл води, колбу з’єднували з паропровідною трубкою. За 5 хв після закінчення перегонки відкривали кран, поступово спускаючи дистилят так, щоб ефірна олія зайняла градуйовану частину трубки приймача, заміряли об’єм ефірної олії. Де: V – об’єм ефірної олії, мл; m – маса сировини, г; W – втрата в масі під час висушування сировини, %. Аналіз компонентного складу ефірної олії плодів лавру благородного визначали за допомогою хромато-мас-спектрометричного методу з використанням газового хроматографа Agilent Technology 6890N, обладнаного мас-селективним детектором 5973N. Газ-носій – гелій; швидкість потоку 1 мл/хв. Компонентний склад ефірної олії ідентифікували методом порівняння хромато-мас-спектрів сполук, які входять у досліджувану суміш, з даними бібліотеки мас-спектрів NIST 02 (понад 174 000 речовин) [22]
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