Abstract
The complete mitochondrial genome of the western honey bee subspecies Apis mellifera meda was sequenced. This mitochondrial genome is 16,248 bp in length, with 37 classical eukaryotic mitochondrial genes and an A + T-rich region. Gene direction and arrangement are similar to those of other Apis mitogenomes. All genes initiate with ATT (six genes), ATG (four genes), ATA (two genes), and ATC (one gene) start codons and terminate with a TAA stop codon. Four genes are encoded on the heavy and nine on the light strands, respectively. All of the 22 tRNA genes, ranging from 66 to 78 bp, have a typical cloverleaf structure. The complete mitogenome of A.m. meda provides information on the biogeography and evolution of A. mellifera subspecies.
Highlights
Apis mellifera meda is a subspecies of western honey bee native to Iran, northern Iraq, and southeastern Turkey (Sheppard 2015)
An adult honey bee worker of A.m. meda was obtained from the Ruttner Bee Collection at the Bee Research Institute at Oberursel, Germany (Voucher No 3284, Pour-Elmi, Iran, 2004, 2713N, 6040E) and its mitogenome is reported (GenBank accession No KY464957)
A genomic library was constructed from the genomic DNA using a Kapa Hyper Prep Kit (Kapa Biosystems, Woburn, MA) with a pairedend read (2 Â 150) followed by next-generation sequencing on the Illumina Hi-Seq 3000/4000 (San Diego, CA)
Summary
Apis mellifera meda is a subspecies of western honey bee native to Iran, northern Iraq, and southeastern Turkey (Sheppard 2015). KEYWORDS Mitogenome; nextgeneration sequencing; Apis mellifera meda Apis mellifera meda shares similarities with A.m. ligustica, A.m. anatolica, and honey bees in northern Iraq (Rahimi & Asadi 2010) and is similar, morphometrically, to A.m. ligustica, the Italian honey bee (Sheppard 2015).
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