Abstract
We sequenced and annotated the species of Acroneuria carolinensis which is the second species representative of the genus Acroneuria to provide mitochondrial genome data for order Plecoptera. The complete mitochondrial genome of A. carolinensis was 15,718 bp in length and possessed 22 transfer RNA (tRNA) genes, 13 protein-coding genes (PCGs), two ribosomal RNA (rRNA) genes and one control region. The A + T account of the whole genome, PCGs, tRNAs, rRNAs, and the control region was 64.6%, 62.7%, 67.8%, 68.6%, and 73.9%, respectively. Ten PCGs used the normal start codon except ND2, ND5, and ND1 used the exceptional GTG and TTG as start codon. All PCGs stopped with the terminal codon TAN, but only COII gene used the single T––. The phylogenetic tree was based on the PCG12R matrix (including PCGs first and second codons and two rRNAs) by Bayesian inference (BI) method and we found that the genus Acroneuria and Sinacroneuria were clustered in a clade.
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