Abstract
The complete nucleotide sequence of Pinus sibirica Du Tour chloroplast genome (cp DNA) was determined in this study. The cpDNA was 116,635 bp in length, containing a pair of 473 bp inverted repeat (IR) regions (IRa and IRb), which were separated by large and small single-copy regions (LSC and SSC) of 63,908 and 51,781 bp, respectively. The cpDNA contained 113 genes, including 81 protein-coding genes, 4 ribosomal RNA genes, and 28 tRNA genes. The majority of these genes were single copy genes, while 4 genes existed as double copies, including 2 protein-coding genes (psbA and ycf2), 2 tRNA genes (trnAP and trnS). The overall GC content of P. sibirica cpDNA is 38.7%, while the corresponding values of the LSC, SSC, and IR regions are 38.0, 39.7, and 37.8%, respectively. The phylogenetic analysis confirmed clusterization of P. sibirica in Strobus subgenus.
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