Abstract
Cucurbita ficifolia Bouché is an important germplasm resource used for rootstock and hypoglycemic food in Cucurbitaceae. The complete chloroplast genome sequence of C. ficifolia has been determined in this study. The total genome size is 157,533 bp in length and contains a pair of inverted repeats (IRs) of 25,639 bp, which were separated by large single copy (LSC) and small single copy (SSC) of 88,112 bp and 18,143 bp, respectively. A total of 130 genes were predicted including 86 protein-coding genes, eight rRNA genes and 36 tRNA genes. Further, Maximum-likelihood phylogenetic analysis revealed that C. ficifolia is a base clade of genus Cucurbita and closer to Cucurbita maxima. The chloroplast genome of C. ficifolia would promote the germplasm exploration, phylogenetic relationships, and molecular biology researches in Cucurbita.
Highlights
Cucurbita ficifolia Bouche is an important germplasm resource used for rootstock and hypoglycemic food in Cucurbitaceae
The fruit of C. ficifolia contains D-chiro-inositol with the hypoglycemic activity in man and other animals (Xia and Wang 2006), is commonly used as a vegetable insulin for remedying diabetes in Asia, Africa and South America (Moya-Hernandez et al 2020), and it seeds have a high content of unsaturated fatty acids, which enables their use as a good and healthy oil to be used in the food industry (Carrillo et al 2018)
The long sequence was taken as complete genome sequence and annotated using CPGAVAS2 and GeSeq (Tillich et al 2017; Shi et al 2019), the problems in annotations was corrected by Sequin
Summary
Cucurbita ficifolia Bouche is an important germplasm resource used for rootstock and hypoglycemic food in Cucurbitaceae. KEYWORDS Cucurbita ficifolia Bouche; chloroplast genome; figleaf gourd The fruit of C. ficifolia contains D-chiro-inositol with the hypoglycemic activity in man and other animals (Xia and Wang 2006), is commonly used as a vegetable insulin for remedying diabetes in Asia, Africa and South America (Moya-Hernandez et al 2020), and it seeds have a high content of unsaturated fatty acids, which enables their use as a good and healthy oil to be used in the food industry (Carrillo et al 2018).
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