The complete chloroplast genome sequence of Pseudolysimachion kiusianum var. diamantiacum (Plantaginaceae)

Abstract

The genus Pseudolysimachion, previously classified as a subgenus of Veronica (Plantaginaceae), was reclassified as an independent genus based on chromosomal and molecular studies. The genus comprises approximately 20 species distributed across Asia and Europe, 16 of which are found on the Korean Peninsula. Among these, Pseudolysimachion kiusianum var. diamantiacum (Nakai) T. Yamaz is an endemic species of the Korean Peninsula, notable for its unique morphological traits. Despite its horticultural potential and existing research on its cultivation, the genetic resources for Pseudolysimachion remain limited. In the present study, we sequenced the complete chloroplast genome sequence of P. kiusianum var. diamantiacum for the first time and conducted a phylogenetic analysis within the genus. The chloroplast genome of P. kiusianum var. diamantiacum is 152,249 bp long and features a large single-copy region (83,187 bp), small single-copy region (17,704 bp), and two inverted repeats (25,679 bp each). Phylogenetic analysis of 35 chloroplast genomes, one genome generated in this study and 34 obtained from the NCBI GenBank confirmed the monophyly of Plantaginaceae and positioned Pseudolysimachion within the Veroniceae tribe. The complete chloroplast genome sequence of P. kiusianum var. diamantiacum provided here can be utilized to develop species-specific markers through comparison with closely related species. Further studies should aim to clarify its phylogenetic position within Pseudolysimachion.