Abstract
The complete chloroplast genome sequence of Daphne tangutica Maxim. (Thymelaeaceae), a medicinal plant distributed in northwest alpine region of China, was characterized the complete nucleotide sequence of chloroplast (cp) genome. The total length of cp genome is 169,944 bp, including a large single copy (LSC) region of 84,884 bp, a small single copy (SSC) region of 2248 bp, and a pair of inverted repeats (IRs) of 41,406 bp. The cp genome of D. tangutica contains 137 genes, including 90 protein-coding genes (75 PCG species), 39 transfer RNA genes (31 tRNA species), and eight rRNA genes (four RNA species). A total of 12 genes (tRNA-Lys (UUU), tRNA-Leu (UAA), tRNA-Val (UAC), tRNA-Ile (GAU), tRNA-Ala (UGC), rpl16, rpoC1, petD, rps16, rpl2, ndhB, and ndhA) contains a single intron, and two genes (ycf3 and rps12) contains two introns. The GC content in whole cp genome, LSC region, SSC region, and IR region were 36.8%, 34.8%, 28.6%, and 38.9%, respectively, which are similar to other Thymelaeaceae plants. Phylogenetic analysis suggested that D. tangutica has the closely reltionship with congeneric D. kiusiana.
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