The complete chloroplast genome of Callicarpa rubella Lindl. (Lamiaceae) from Guangxi, China: genome structure and phylogenetic analysis

Huimin Cai,Xing Liu,Zhiwei Su,Zhonghui Ma

doi:10.1080/23802359.2021.1993106

Huimin Cai, Xing Liu + Show 2 more

Open Access

https://doi.org/10.1080/23802359.2021.1993106

Copy DOI

Abstract

The relationship between Callicarpa rubella Lindl. and its infraspecific taxa has troubled researchers for a long time. Here, we reported for the first time the complete chloroplast (cp) genome of C. rubella to investigate its phylogenetic position and provide more sequencing information for further studies about the relationship between C. rubella and its related species. The cp genome of C. rubella was 154,202 bp in length and displayed a typical quadripartite angiosperm structure, containing two inverted repeat (IR) regions of 25,701 bp, a large single-copy (LSC) region of 84,968 bp and a small single-copy (SSC) region of 17,832 bp. It contained 87 protein-coding genes, 37 tRNA genes and 8 rRNA genes. The analysis fully resolved C. rubella was in a clade with C. bodinieri and C. nudiflora. The results indicated Callicarpa formed a sister relationship with Dicrastylis parvifolia in Lamiaceae.

Highlights

The relationship between Callicarpa rubella Lindl. and its infraspecific taxa has troubled researchers for a long time
The cp genome was assembled by GetOrganelle toolkit (Bankevich et al 2012; Langmead and Salzberg 2012; Wick et al 2015; Jin et al 2020) and annotated using Plastid Genome Annotator (PGA) (Qu et al 2019)
We recovered a total of 128 distinct genes, including 87 protein-coding genes (PCGs), 37 tRNA genes and 8 rRNA genes

Summary

Introduction

The relationship between Callicarpa rubella Lindl. and its infraspecific taxa has troubled researchers for a long time. KEYWORDS Callicarpa rubella; chloroplast genome; Lamiaceae; phylogenetic relationship We assembled and annotated the complete chloroplast (cp) genome of C. rubella for the first time to serve as a genetic resource for future studies on the taxonomy of Callicarpa and to get a better understanding of phylogenetic relationships in this pantropical genus.

Results

Conclusion