Abstract
Rat liver Golgi apparatus was prepared from the mitochondrial fraction after mild homogenization (stainless-steel sieving), and was subfractionated by sucrose density gradient centrifugation into light (Mt-GF1), intermediate (Mt-GF2), heavy (Mt-GF3) and precipitate (Mt-ppt) subfractions. These were compared with the corresponding subfractions prepared from the microsomal fraction of the same homogenate (Ms-GF1, Ms-GF2, Ms-GF3 and Ms-ppt). 1. 1. The content of serum albumin and the activity of acid phosphatase were much higher in subfractions isolated from the microsomal fraction than in those from the mitochondrial fraction. 2. 2. Radioactive inorganic iron administered intravenously into the animals was preferentially incorporated into Ms-GF2. 3. 3. Electronmicroscopic observations revealed that vesicles containing very low density lipoprotein particles in Ms-GF2 were large in size and round in shape, whereas those in Mt-GF2 were small and heterogeneous with many distorted profiles, such as rod- or doughnut-like structures. 4. 4. The results demonstrated that Golgi elements prepared from the mitochondrial fraction differed in their biochemical and morphological properties from those prepared from the microsomal fraction. We suggested that these elements were derived from different parts of the Golgi apparatus, the former from the less mature region of the organelle and the latter from the mature region. It was also suggested that the mature region was more susceptible to a mechanical shearing force.
Published Version
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