The comparison of curative effects between mesenchymal stem cells of the human umbilical cord and mesenchymal stem cells of rat bone marrow in rats with spinal cord injuries

Abstract

Objective To observe changes in the functional recovery and immunological rejection of rats after spinal cord injuries with the treatment of transplanting human umbilical cord mesenchymal stem cells and rat bone marrow mesenchymal stem cells,to find the right stem cells for clinical procedures through this experiment,and to investigate the mechanism of immunological rejection after transplanting the cells.Methods Forty SD rats were selected randomly and divided into 3 groups.16 rats were placed in the umbilical cord mesenchymal stem cell transplantation group,16 rats in the bone marrow mesenchymal stem cell transplantation group,and 8 rats in the PBS group.The levels of 1I-2 and IL-10 in the blood of each group were detected at different times (1W and 2M,and 3 months after the cell transplantation) to reflect the systemic immunological rejection of the goat after transplantation.The CD3 + cells in the cell transplantation areas of each group were also detected with immunohistochemistry to reflect local immune rejection after transplantations.Results 1.The level of IL-2 was obviously higher than the PBS group lW and 2M and 3 months after the cell transplantation (P ＜0.05).Meanwhile,IL-10 was Contrary to the IL -2.2.The level of IL-2 was obviously higher than the PBS group 1W and 2M and 3 months after the cell transplantation (P ＜ 0.05).Meanwhile,IL-10 was Contrary to the IL-2.3.The quantity of CD3+cells in all groups were much at the acute period (1 W after cell transplantation),but only the quantity of CD3+cells in the bone marrow mesenchymal stem cell transplantation group was much larger compared to the other groups at the chronic period 3 months after cell transplantation (P ＜ 0.05).Conclusion Systemic and local immunological rejections at acute or chronic periods will appear in rats with spinal cord injuries that underwent stem cell transplantations.Compared with bone marrow mesenchymal stem cells,the umbilical cord mesenchymal stem cells significantly have lower immunogenicity and more suited for functional recoverv. Key words: Spinal cord injury; Umbilical cord mesenchymal stem cells; Bone marrow mesenchymal stem cells; Immunological rejection; Functional recovery