THE COMPARISON BETWEEN DIFFERENT ENRICHMENT BROTH MEDIA AND SELECTIVE SOLID MEDIA FOR GROWING OF Salmonella typhimurium AND Listeria monocytogenes

Abstract

The effect of different enrichment media i.e brain heart infusion (BHI broth), tryptic soy broth (TSB), nutrient broth (NB), buffered peptone water (BPW), university of Vermont medium (UVM1) and Listeria enrichment broth (LEB) on the growth of Salmonella typhimurium and Listeria monocytogenes was stueid. Plating selective media as well as bismuth sulfate, brillient green and xylose lysine deoxycholate (XLD) was also evaluated for isolation of Salmonella typhimurium. Effect of modified buffered peptone water for growth of Salmonella typhimurium was studied. The influence of acriflavine and nalidixic acid on the growth of Listeria monocytogenes was also evaluated. The results revealed that the best enrichment medium for Salmonella typhimurium was TSB followed by BHI broth, while BPW was the lowest enrichment medium for Salmonella typhimurium. On the contrary BPW was the most suitable one for enrichment of Listeria monocyto genes. Bismuth sulfate was the most suitable for isolation of Salmonella typhimurium. Modification of dextrose or lactose content (or percent) paid to the ability increasing of BPW for enrichment of Salmonella typhimurium. The addition of acriflavine and nalidixic acid to TSB and BHI broth had not the ability to enrichment of L. monocytogenes. On the contrast unaddition acriflavine and nalidixic acid to LEB and UVM1 had the ability to enrichment of L. monocytogenes.