Abstract

The genomic islands (GIs) are usually the products of horizontal gene transfer (HGT) that is evolution pattern in prokaryote. Two homologous GIs (WSU1GINoand KU01GINo) containing the homologous integrase of Bacteriophage P2 were determined inEnterobacter cloacaethrough flanking sequence alignment of the homologous integrase. The homologous GIs were integrated into the noncoding sequence. Their common flanking sequence is 5-AAGGCTCCCTCAGGAGC-3, and their integrases share 97% similarity. About two-thirds of the nucleotide sequences between WSU1GINoand KU01GINoare highly similar. The different regions between WSU1GINoand KU01GINomainly include hypothetical gene, Phage-related tail gene, capsid gene, and baseplate assembly gene. In conclusion, the tandem arrangement of WSU1GINoand KU01GINowill be artificially constructed because of their similar structural characteristics, and phage coat protein assembly will also be analyzed.

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