The Combined Effects of Nano-Zinc, Nano-Albumin and Honey in Healing Wounds Caused by Third-Degree Burn in Male Mice

Abstract

Background: There is extensive evidence on the healing properties of honey. Therefore, in this study the effect of nano zinc and nano-albumin combination in topical treatment of third-degree burn was evaluated. Methods: In this experimental study, 16 adult male mice were randomly divided to experimental group 1 (treated with honey and nano-zinc), experimental group 2 (treated with nano-albumin and honey), experimental group 3 (treated with honey, nano-albumin, and nano-zinc), and group 4 (no treatment). Under sterile conditions and using general anesthesia, third-degree burns on the back of each mouse was created. Topical burn treatment was performed once a day for 6 weeks, and after the end of treatment, the mice were scarified by anesthesia and then a sample of wound bed of mice was prepared and fixed in formalin. A histological process was performed on the samples. The specimens were stained with H and E staining and microscopic observations were made. Results: At the macroscopic level, after the treatment of burn wounds, shrinkage of burn wound and scar reduction was observed in honey and nano-zinc treated group compared to the control and other treated groups. Histological studies showed a significant increase in total thickness of dermal and epidermal layers of skin in this experimental group compared to other groups (P < 0.05). Discussion: The results showed that the combination of organic honey and nano-zinc could accelerate the healing of burn wounds in mice, while adding nano-albumin to the mixture had opposite effects and could delay wound healing.