The Collagen Origin Influences the Degradation Kinetics of Guided Bone Regeneration Membranes.

Marta Vallecillo-Rivas,Raquel Osorio,Manuel Toledano-Osorio,Manuel Toledano,Cristina Vallecillo

doi:10.3390/polym13173007

Marta Vallecillo-Rivas, Raquel Osorio + Show 3 more

Open Access

https://doi.org/10.3390/polym13173007

Copy DOI

Abstract

Collagen membranes are currently the most widely used membranes for guided bone regeneration; however, their rapid degradation kinetics means that the barrier function may not remain for enough time to permit tissue regeneration to happen. The origin of collagen may have an important effect on the resistance to degradation. The aim of this study was to investigate the biodegradation pattern of five collagen membranes from different origins: Biocollagen, Heart, Evolution X-fine, CopiOs and Parasorb Resodont. Membranes samples were submitted to different degradation tests: (1) hydrolytic degradation in phosphate buffer saline solution, (2) bacterial collagenase from Clostridium histolyticum solution, and (3) enzyme resistance using a 0.25% porcine trypsin solution. Immersion periods from 1 up to 50 days were performed. At each time point, thickness and weight measurements were performed with a digital caliper and an analytic microbalance, respectively. ANOVA and Student–Newman–Keuls tests were used for comparisons (p < 0.05). Differences between time-points within the same membranes and solutions were assessed by pair-wise comparisons (p < 0.001). The Evolution X-fine collagen membrane from porcine pericardium attained the highest resistance to all of the degradation tests. Biocollagen and Parasorb Resodont, both from equine origin, experienced the greatest degradation when immersed in PBS, trypsin and C. histolyticum during challenge tests. The bacterial collagenase solution was shown to be the most aggressive testing method.

Highlights

Nowadays, both in oral implantology and periodontology, the management and treatment of alveolar deficiencies are major clinical issues
Guided bone regeneration (GBR), a technique based on the use of membranes creating space to be filled with new bone, was originally hypothesized more than three decades ago [1,2]
Resorbable membranes are used more frequently for GBR, alleviating the discomfort of the patient caused by a second surgery, avoiding tissue damage and the risk of additional morbidity [7]

Summary

Introduction

Both in oral implantology and periodontology, the management and treatment of alveolar deficiencies are major clinical issues. Guided bone regeneration (GBR), a technique based on the use of membranes creating space to be filled with new bone, was originally hypothesized more than three decades ago [1,2]. This concept implies the exclusion of those cells types that proliferate faster than bone cells, such as those destined to form epithelial and connective tissue [3,4]. Non-resorbable membranes remain stable over time without undergoing degradation processes On the contrary, these membranes require a second surgery for their removal [8,12]. Among the different resorbable membranes available, collagen membranes are the most widely studied and investigated [6]

Objectives

Methods

Results

Discussion

Conclusion