Abstract
Native collagen-based membranes are used to guide bone regeneration; but due to their rapid biodegradation, this treatment is often unpredictable. The purpose of this study was to investigate the biodegradability of natural collagen membranes. Three non-cross-linked resorbable collagen barrier membranes were tested: Derma Fina (porcine dermis), Evolution Standard (equine pericardium) and Duo-Teck (equine lyophilized collagen felt). 10 × 10 mm2 pieces of membranes were submitted to three different degradation procedures: (1) hydrolytic degradation in phosphate buffer solution, (2) enzyme resistance, using a 0.25% porcine trypsin solution, and (3) bacterial (Clostridium histolyticum) collagenase resistance test. Weight measurements were performed with an analytic microbalance. Thickness was measured with a digital caliper. Membranes were analyzed at different time-points, up to 21 d of immersion. A stereomicroscope was used to obtain membranes’ images. ANOVA and Student Newman Keuls were used for mean comparisons (p < 0.05), except when analyzing differences between time-points within the same membrane and solution where pair-wise comparisons were applied (p < 0.001). Derma Fina attained the highest resistance to all degradation challenges. Duo-Teck was the most susceptible membrane to degradation, complete degradation occurred as soon as 8 h. The bacterial collagenase solution performed as the most aggressive test as all membranes presented 100% degradation before 21 d.
Highlights
The aging population is the principal reason to advance the understanding of dental biomaterials aimed to achieve periodontal regeneration [1]
guide bone regeneration (GBR) membrane demonstrated the greatest resistance to the different degradation challenges under in vitro conditions (Tables 1 and 2; Figures 1 and 2)
Both Evolution Standard and Duo-Teck membranes have a similar composition that is GBR membrane demonstrated the greatest resistance to the different degradation challenges under in vitro conditions (Tables 1 and 2; Figures 1 and 2)
Summary
The aging population is the principal reason to advance the understanding of dental biomaterials aimed to achieve periodontal regeneration [1]. Restoration of the lost periodontium is a paramount goal of periodontal therapy [2], where the guide bone regeneration (GBR) concept plays a capital roll. GBR is a surgical procedure that uses membranes as barriers to prevent the ingrowth of fibroblasts and to maintain a space for osteogenesis. The use of a barrier membrane to promote the selective repopulation of a periodontal/bone defect by cells with regenerative potential has been successfully applied for more than 40 years when the first application of a cellulose acetate laboratory filter by Millipore for GBR occurred [3]. GBR is a method successfully employed in dental practices aimed to increase the volume of the host bone at sites with insufficient bone quantity [4]
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