The clinical significance of the relationship between serum lost goodwill target proteome and the acute physiology and chronic health evaluation II score of patient with critical illness

Abstract

Objective To investigate the expression of serum lost goodwill target(LGT)proteome,and to analyze its clinical significance in evaluating prognosis of patient with critical illness on the basis of acute physiology and chronic health evaluation Ⅱ(APACHE Ⅱ)score. Methods The serum samples were collected from 96 patients with critical illness and 30 healthy volunteers as healthy control. The expression of serum LGT proteome was detected by surface-enhanced laser desorption/ionization-time of flight-mass spectrometry(SELDI-TOF-MS)protein-chip technology. The abundance value of LGT proteome in patients at admission was measured, and at the same time APACHE Ⅱ score was estimated, in order to analyze its clinical significance in patients with critical illness. Results The amount of LGT proteome in APACHE Ⅱ≥15 group[n= 35,(9.26 ± 7. 51)%]was significantly higher than that of APACHE Ⅱ＜ 15 group[n= 61,(4. 19 ± 4.07)%], and the LGT proteome amount in both groups was significantly higher than that of the healthy control group[(1.52± 0.47)%, both P＜0.01]. Spearman correlation analysis showed that there was significant positive correlation between the abundance of LGT proteome and the APACHE Ⅱ score (r=0. 317, P=0. 002). The abundance of LGT proteome in death group[n=23,(10. 14±9. 23)%]was significantly higher than that in survival group[n=73,(5. 83±3.57)%, P＜0. 01]. The fatality rate of the LGT proteome group with average abundance exceeding 5%[68.0%(17/25)]was significantly higher than that of the LGT proteome group with average abundance lower than 5%[8.5%(6/71), P＜0.01].According to the LGT proteome abundance to evaluate the prognosis of the patients, the positive predict rate was 68.0 %, the negative predict rate was 91.5 %, the false positive rate was 32. 0%, the false negative rate was 8.5%. Conclusion The LGT proteome was intimately correlated with the severity degree of disease condition and prognosis in patients with critical illness. The determination of LGT proteome combined with APACHE Ⅱ score evaluation can probably be an important indicator in evaluating the prognosis of patient with critical illness. Further research on LGT proteome is warranted to facilitate the prognostication and clinical decision-making. Key words: Critical illness ;  Acute physiology and chronic health evaluation Ⅱ ;  Lost goodwill target proteome ;