Abstract
The nuclear DNA content of paraffined-embedded surgical biopsies from 84 patients with non-Hodgkin's lymphoma was measured by flow cytometry. DNA aneuploidy was detected in 32 cases (38%) and was not related to histologic grade. The incidence of aneuploidy did not correlate with survival. S-phase fraction was significantly higher in morphologically high grade and intermediate grade than in low-grade (P < 0.05) . The percentage of S-phase cells was found to be a valuable prognostic parameter using a cut point at 18 percent. The S-phase values below 18 percent had a significantly better survival than those with S-phase values above 18 percent (P<0.05) .We considered that the flow cytometric DNA analysis revealed characteristic features in non-Hodgkin's lymphomas, and the S-phase value might be useful in predicting the clinical outcome of patients. Key words: Non-Hodgkin's lymphoma; DNA analysis, flow cytometry; paraffinedembedded samples
Published Version
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