Abstract
This research was aimed at discovering the serological and histological changes in cardiac and hepatic tissue after electric shock. The CK-MB, ALT, and AMS indexes were tested with serological methods. Moreover, the Bcl-2, Bax, and Hsp-60 expression levels were carefully measured. An electrical injury model was established by giving rats electric shocks at 110 V with alternating electric current. Blood samples from the rats were analyzed for the biochemical indexes. The degrees of pathological changes in the heart and liver were evaluated using IHC staining for Bcl-2, Bax, and Hsp-60. The levels of CK-MB in the electrical injury group rapidly peaked at 0.5 hours after the electric shock. Additionally, the levels of Bcl-2, Bax, and Hsp-60 in the cardiac and hepatic tissues changed regularly after the electrical injury and exhibited apparent differences from the levels in the control group. CK-MB, ALT, and AMS were altered regularly after electric shock, and these results provide significant information for clinical and medicolegal practice. This research has shed light on the assessment of electrical injury without obvious electrical burns. Furthermore, the findings obtained for Bcl-2/Bax and Hsp-60 can also facilitate pathological diagnosis and the identification of antemortem and postmortem electrical injury.
Highlights
Injury caused by electrical current, known as electrical injury, can occur and frequently due to the widespread use of electrical appliances [1, 2]
We studied the changes in Bc1-2/Bax and Hsp by pathological detection and examined creatine kinase-MB (CK-MB), alanine transaminase (ALT), and amylase (AMS) by using serological methods to evaluate the degree of injury after electric shock
We proved the existence of organ injury after electrical injury and the regularity of repairs
Summary
Injury caused by electrical current, known as electrical injury, can occur and frequently due to the widespread use of electrical appliances [1, 2]. The inability to diagnose whether the injuries were caused by electricity may impede the treatment of the patients or investigations of deaths [1,2,3,4,5,6]. An easy method to detect electrical injury is necessary. Many studies have proved that electric shocks cause multisystem injuries and produce secondary regular variations at the cellular and molecular levels. Detecting the molecules in some particular organs and some enzymes may represent alternative methods to help diagnose and evaluate electrical injuries. These enzymes, including CK-MB, ALT, and AMS, provide an indirect index of injuries or illnesses in organs. Hsp is an important member of the Hsp family, which is a group of highly conserved proteins that function in the dislocating, folding, and assembling of proteins as molecular chaperones
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