Abstract
This work was carried out to analysis of immune genes (Lectin (245 bp), PoPO (121 bp), BGBP (166 bp), hemocyanin (242 bp), Toll receptor (150 bp) and immunological analysis in white shrimp (Litopenaeus vannamei) infected with WSSV and herbal immune stimulant (immuzone) treated. And also to understand the level of expression and distribution of Heat Shock Proteins (Hsp) in white shrimp (Litopenaeus vannamei). Under untreated condition, all the immune genes (Lectin, PoPO, BGBP, hemocyanin, Toll receptor) were differentially expressed in all of the examined tissues. Under WSSV infected and immuzone treated condition, immune genes were inducible in all tissues when compared to its untreated condition. The expression levels of Hsp21, Hsp70 and Hsp90 were determined by quantitative real-time PCR in four tissues (gill, hepatopancreas, pleopod and muscle) of Litopenaeus vannamei in WSSV treated and normal shrimps. Under untreated condition, all three Hsp genes were differentially expressed in all of the examined tissues. Under WSSV infected condition, only Hsp70 was inducible in all tissues when compared to its untreated condition. The time course induction experiment in gill, muscle, pleopod and hepatopancreas revealed that the transcriptional level of Hsp70 was induced and that Hsp21 and Hsp90 were uninducible under the WSSV treated condition. The expression level of Hsp70 was significantly increased after a 24, 48-h exposure to WSSV whereas the Hsp21 and Hsp90 transcripts were down regulated later after WSSV exposure. This evidence suggests that there is a putative role and involvement of the Hsp genes as a part of immunity response against WSSV in Litopenaeus vannamei.
Highlights
Aquaculture industry is one of the major economic resources of many countries
Transcriptional analysis of heat shock proteins (Hsp21, 70 and 90) showed that in White Spot Syndrome Virus (WSSV) injected shrimps, Hsp21 was down regulated in all experimental organs (Figure 1), whereas Hsp70 was up regulated in all experimental organs with different time course after WSSV injection (Figure 2)
To assess a time-dependent induction of the Heat Shock Proteins (Hsps) gene expression, Cycle Threshold (CT) values of the Hsp21, Hsp70, Hsp90 and ef1α transcripts were measured in L. vannamei exposed to the WSSV infection by real-time Polymerase Chain Reaction (PCR) (Tables 2-5)
Summary
For the last three decades it is being threatened by many pathogens, especially viruses. Among these viruses, the White Spot Syndrome Virus (WSSV) is the most serious one. The molecular mechanisms underlying the majority of antiviral responses in shrimps are still unknown and are only beginning to be addressed [1]. Shrimp molecular responses to viral pathogens have been reviewed by Flegel and Sritunyalucksana [2] as innate immune response comprising the humoral interactions (Toll pathway, proPO and Clotting system, Antioxidant enzymes, Lectins, Haemocyanin, etc.) and the cellular responses (Apoptosis pathway, Jak-STAT pathway, RNAi pathway). Studies to investigate the host immune response after WSSV infection and prophylactic/therapeutic methods have become necessary [3,4,5,6]. WSSV structural proteins such as VP19, VP28 and VP26 have been adopted to develop a prophylactic vaccine against WSSV disease [7,8]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
