Abstract
A method for the standardization of the crustacean eye-stalk hormone on the blinded fiddler crab, Uca, has been described. The Uca unit has been defined as the amount of hormone contained in 1 cc. of solution, 0.05 cc. of which when injected into each of 15 specimens of Uca pugilator blinded 2 days previously produces a response whose average duration is about 5.0 hours. The response is measured as the amount of time intervening between the injection of the hormone and the time at which the animals again become pale, an interval during which the melanophores expand, remain expanded for some time, and finally contract. The hormone content in the eye-stalks of various crustaceans was determined. A method for the extraction and purification of the hormone has been described, and some chemical and physical properties of the hormone have been listed.The amount of hormone extracted from the eye-stalks of Palaemonetes is the same regardless of whether the hormone is secreted continuously into the circulation, or ...
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