The chick embryo nodose ganglion: effects of nerve growth factor in culture.

Abstract

Nodose ganglia from 8-day-old chick embryos were cultured in collagen gels for 2 days, with or without added nerve growth factor (NGF), in order to discover whether the nodose neurons, derived from an epidermal placode, are susceptible to this trophic factor. Neuronal survival and neurite outgrowth were stimulated only after addition of NGF, and the enhancing effects could be blocked by introducing antibodies to NGF. Stereological analysis of ganglia sectioned for light microscopy showed that the NGF-treated neurons increased their volume by about 50%, as did the nodose neurons in ovo from the eighth to the tenth day of incubation. The volume density, however, was significantly lower in vitro indicating a limited cell death during culture despite the presence of exogenously supplied NGF. The number of neurofilaments and microtubules increased in the cell centre of neurons treated with NGF; this region also showed numerous dense bodies and an extensive Golgi complex by electron microscopy. Ultrastructural similarities between neurons responding to NGF and neurons undergoing the axon reaction which follows axotomy are indicated. A role for a trophic factor resembling NGF in the normal development of placode-derived neurons of the sensory cranial ganglia is suggested.