Abstract
A method has been devised for the separation by mild methods of the orthocortical and paracortical cells of wool. It consists of preferential deposition of gold in the paracortex, disruption of the fiber by ultrasonication in formic acid and separation of the more heavily stained paracortical cells from the orthocortical cells by centrifugation in a density gradient. Amino acid analysis shows that the orthocortical cells contain more tyrosine (26%), glycine (16%), leucine (12%), phenylalanine (12%) and less (cystine/2 + cysteic acid) (14%) and histidine (14%) than the paracortical cells. These relatively small differences in amino acid composition are unlikely to account for the considerable differences in the properties of the two cortices.
Published Version
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