Abstract
The objective of this workshop was for investigators to characterize the idiotype systems they were employing to evaluate the expression of common idiotype families on the immunoglobulins and anti-DNA antibodies of patients with systemic lupus erythematosus (SLE) as well as other diseases, and in normal individuals. Twenty-four different systems were described, in which the idiotypes identified were expressed in SLE or related disorders (see Table). Each investigator was asked to provide information on the nature and source of the anti-idiotype probes employed to search for the expression of each idiotype amongst other antibodies, and the location (where known) for each idiotype on the heavy or light chain variable region, including any data on the molecular localization of idiotypic determinants. The 16/6 idiotype family and its expression in various systems was discussed by several groups. The prototype SLE peripheral-blood derived hybridoma monoclonal IgM kappa anti-DNA antibody employed to generate anti-16/6 idiotype probes showed higher binding to poly (I), poly (dT) and denatured DNA than to native DNA; it also showed binding to cell membrane antigens (platelets and lymphocytes), glycolipids extracted from the cell membrane of Mycobacteria tuberculosis, Klebsiella polysaccharide K30, and cytoskeletal structures. The expression of this idiotype was probed by a rabbit anti-16/6 polyclonal antibody, and in previous studies in SLE sera, 50% of patients with active disease expressed this idiotype on serum immunoglobulins as well as on 40% of immunoglobulins deposited in SLE skin and kidney lesions. The 16/6 Id has also been identified on paraproteins of G, M and A isotypes and in the serum of patients with mycobacterial infections during the active stages of their disease. Dr Lori Tucker (Boston) described some of the studies she is currently undertaking in Dr Schwartz's laboratory, looking at the molecular aspects of 16/6 expression. The parent 16/6 IgM~c antibody utilizes an Ig heavy chain variable region gene from the VHuI family, and the heavy chain of 16/6 is responsible for the expression of
Published Version
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