Abstract
Objective To prepare the rat model of type 2 diabetes mellitus (T2DM), and to observe the characteristics of peripheral neuropathy. Methods High fat and high sugar diets were fed for 8 weeks to induce insulin resistance and then low dose streptozotocin (STZ) was injected intraperitoneally to induce type 2 diabetes mellitus models in Sprague Dawley rats. Blood glucose and serum insulin levels continuous were monitored. Tactile allodynia in response to von Frey (VF) filament stimulation of the plantar hind paws and paw withdrawal thermal latency (PWTL) to plantar test were used as the criterion for diabetic neuropathy. Instruments AD was used to detect nerve conduction velocity (NCV) of sciatic nerve in rat and the morphological and pathological changes of sciatic nerve were detected by electron microscope. Results The characteristics of T2DM rats by peripheral neuropathy in this method were that 50% force withdrawal threshold and PWTL were measured. Both values of diabetic rats were decreased from the day of STZ injection until 4 weeks after STZ injection, and then increased 8 weeks after STZ injection (50% force withdrawal threshold values, (11.8±0.8)g, (8.4±0.7)g and (16.2±1.4)g; PWTL (10.2±0.9)s, (8.3±1.2)s and (13.2±1.0)s. These results indicated that tactile sensation changed from hypersensitive to hyposensitive. Compared to the NC group, the sciatic nerve motor and sensory conduction velocity were significantly decreased at 4 and 8 weeks in DM group, respectively. Compared to DM group at 4 weeks, the sciatic nerve motor and sensory conduction velocities were further decreased in the DM group at 8 weeks. Conclusively, sciatic nerve showed obvious demyelination and axonal collapse. Conclusions T2DM rat model was successfully induced by high fat and sugar diet combined with small dose of STZ injection. The rat model has typical pathological change of peripheral nerve. It might provide a particularly advantageous tool for investigations of diabetes and its chronic complications. Key words: Diabetes mellitus, experimental/CO; Peripheral nervous system diseases/CO; Streptozocin/AD
Published Version
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