Abstract
Objective To analyze the change of CD4+ CD25 + regulatory T cells in peripheral blood in rabbit' s lung VX2 tumor resection model. Methods Thirty New Zealand rabbits were divided into 3 groups: group A, 10 rabbits with lung VX2 tumor receiving lobectomy; group B, 10 rabbits with lung VX2 tumor receiving no operation; group C, 10 rabbits without lung VX2 tumor receiving lobectomy only.The peripheral blood of rabbits was drawn. All the samples were analyzed by flow cytometry (FCM). Results The proportion of the CD4 + CD25 + regulatory T cells in peripheral blood of the rabbits was increased after the implantion of VX2 tumor ( P ＜ 0. 01 ), that in the rabbits with VX2 lung tumor receiving tumorectomy was obviously decreased at the 15th and 30th day after the operation (P ＜ 0. 05 ), but that in the rabbits with VX2 lung tumor receiving no operation did not have the same change ( P ＜ 0. 05 ). Conclusion The increase of the proportion of CD4 + CD25 + regulatory T cells in peripheral blood is related to immunosuppression. The functional disorder of immune system recovers in a certain extent after resection of the tumor. Key words: Lung tumor; Regulatory T cells; Operation; Flow cytometer
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