Abstract
The effects of exogenous ash (EA) from harvest wheat straw and its internal components on wheat straw autohydrolysis efficiency and subsequent enzymatic hydrolysis were investigated. Results showed that when EA and its insoluble mineral components were included in the autohydrolysis, the enzymatic efficiencies of pretreated residues were significantly reduced from 84.9% to 66.3% and 58.4%, respectively. This was found to be largely attributable to the buffering of free H+ in the pretreatment medium which took place due to the ash. Specifically, the insoluble mineral fraction of said ash exerted strongest buffering capacity in EA. Furthermore, this decrease was found to linearly correlate with decreases to substrate enzymatic accessibility and hydrophobicity. These results demonstrate that the penalties of ash upon autohydrolysis are borne of specific fractions comprising the ash, making the case for ash removal processes or supplementation of processes with additives that will counter the negative effects of ash.
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