The cerebellar and vestibular nuclear complexes in the turtle. I. Projections to mesencephalon, rhombencephalon, and spinal cord.

Abstract

Cerebellar and vestibular projections were investigated in the turtle Pseudemys scripta elegans following injection of 35S-methionine into the cerebellar and vestibular nuclear complexes at various locations. Fibers arising from the cerebellar nuclei were traced via the cerebellar commissure to the contralateral vestibular nuclear complex (particularly the n. vestibularis inferior and n. vestibularis ventrolateralis) and caudal rhombencephalic tegmentum. Ascending projections crossing the midline in the ventral isthmomesencephalic tegmentum terminated in the contralateral red nucleus and nuclei of the fasciculus longitudinalis medialis (f lm). Vestibular projections ascending mainly via the f lm terminated in the nuclei of the f lm, the nuclei of the posterior commissure, and particularly the extraocular motor nuclei. Vestibulo-ocular projections arising from the rostral vestibular nuclear complex were almost exclusively ipsilateral; those from the caudal vestibular nuclear complex were bilateral. Evidence for a topographic organization of the projections to the trochlear and oculomotor nuclei was also obtained. There were some vestibular projections to the contralateral rhombencephalic tegmentum and n. vestibularis inferior. Spinal projections coursing within the ipsilateral ventral descending tract and the ipsilateral fasciculus longitudinalis medialis were found to arise from both rostral and caudal vestibular regions. The caudal vestibular nuclear complex in addition gave rise to fibers descending in the contralateral fasciculus longitudinalis medialis. Evidence for the existence of labeled fibers crossing at spinal levels was also obtained. Vestibulospinal terminations appeared restricted to the ventral horn.