The cephalosporin-resistant bacterial strains from urinary tract

Abstract

Urinary tract infections are considered of great relevance among infections of bacterial origin as one of the third most important bacterial infections worldwide. These infections are caused by bacteria belonging to the Enterobacteriaceae family, such as Escherichia coli, which is the most frequently isolated uropathogen. The objective was to determine the level of resistance to cefotaxime and ceftazidime in bacterial strains from urinary tract infections. Material and methods. The study strains were isolated from patients who presented urinary tract infections, the genus and species were ratified by conventional microbiological methods. The antimicrobial sensitivity test was performed using the Kirby Bauer method and minimum inhibitory concentration, testing β-lactams that belong to the family of third-generation cephalosporins (cefotaxime and ceftazidime). The presence of extended-spectrum β-lactamases was searched for by the combined disc method. Forty-three bacterial strains from a collection of strains from the Microbiology Laboratory of the Faculty of Chemical Sciences, BUAP, were analyzed. All the strains studied showed resistance to the antimicrobials tested and production of the extended-spectrum β-lactamase enzyme. This study showed that E. coli continues to be the most frequent uropathogen responsible for urinary tract infections. It also showed the participation of resistance mechanisms, such as the presence of extended-spectrum β-lactamases, which confer high resistance values ​​to third generation cephalosporins, reflecting the need to implement measures for the use of antimicrobials in our community.