Abstract
Objective To discuss different stomach trouble change occurred in the process of development until the progression in patients with c-myc mRNA expression in correlation with helicobacter pylori (Hp) infection and its relationship with gastric tissue programmed cell death.Methods By real-time fluorescent quantitative reverse transcription-olymerase chain reaction (FQ-PCR) to detect 72 cases of chronic superficial gastritis (CSG),55 cases of chronic atrophic gastritis (the CAG),52 cases of intestinal metaplasia (IM),46 cases of atypical hyperplasia (AH),65 cases of gastric cancer (GC) group (CSG group,respectively,the CAG group,IM,AM group,AH,the GC group) in the c-myc mRNA expression;Rapid enzymatic urea,Warthin Starry silver staining and toluidine blue staining assay for gastric mucosa Hp infection;In situ end labeling (TUNEL) method to detect gastric mucosal cell apoptosis index (AI).Results CSG group,the CAG group,IM,AM group,AH,the GC group c-myc in gastric mucosa tissue mRNA positive example were 21.5％ (7/31),31.0％ (8/27),41.0％ (10/26),59.1％ (14/24) and 69.1 (23/33),c-myc relative mRNA expression levels were 0.089 ± 0.002,0.01 13 ± 0.014 mm,0.168 ±0.028,0.226,0.367 ±0.037 mm,0.040 mm IM,AH group,GC group c-myc amount of mRNA expression were higher than in CSG,the CAG group (P ＜ 0.01),the AH group is higher than the IM group (P ＜0.01),the GC group higher than IM,AH group (P ＜0.01).C-myc mRNA expression quantity and negatively correlated with gastric mucosa cells AI (rs =-0.700,P ＜0.05) ;IM,AH group,GC group Hp positive patients with gastric mucosa cell c-myc mRNA expression were higher than Hp negative patients (P ＜0.05 or 0.01).Conclusion In the process of the gastric mucosal canceration,Hp infection can increase gastric mucosa cell c-myc mRNA expression,and inhibition of special-shaped programmed cell death (apoptosis) and play a role of cancer. Key words: C-myc mRNA ; Tumor suppressor gene; Apoptosis
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