Abstract
ObjectivesArachnids have fascinating and unique biology, particularly for questions on sex differences and behavior, creating the potential for development of powerful emerging models in this group. Recent advances in genomic techniques have paved the way for a significant increase in the breadth of genomic studies in non-model organisms. One growing area of research is comparative transcriptomics. When phylogenetic relationships to model organisms are known, comparative genomic studies provide context for analysis of homologous genes and pathways. The goal of this study was to lay the groundwork for comparative transcriptomics of sex differences in the brain of wolf spiders, a non-model organism of the pyhlum Euarthropoda, by generating transcriptomes and analyzing gene expression.Data descriptionTo examine sex-differential gene expression, short read transcript sequencing and de novo transcriptome assembly were performed. Messenger RNA was isolated from brain tissue of male and female subadult and mature wolf spiders (Schizocosa ocreata). The raw data consist of sequences for the two different life stages in each sex. Computational analyses on these data include de novo transcriptome assembly and differential expression analyses. Sample-specific and combined transcriptomes, gene annotations, and differential expression results are described in this data note and are available from publicly-available databases.
Highlights
Given that spiders have unique sex-specific behaviors and that progress is ongoing in developing arachnid genomics, our goal was to generate transcriptomes and gene expression data using Messenger RNA (mRNA) from brains of males and females of the wolf spider, Schizocosa ocreata
Data description mRNA was isolated from brain samples of immature (Imm; subadult) and mature (Mat; adult) male and female Schizocosa ocreata, collected in Lancaster County, Nebraska
Illumina paired-end sequencing was performed with libraries generated from mRNA derived from individual brain samples, with three replicates for each sex/ stage (Data set 1; National Center for Biotechnology Information (NCBI) Sequence Read Archive (SRA): SRP302932)
Summary
Data description: To examine sex-differential gene expression, short read transcript sequencing and de novo transcriptome assembly were performed. Computational analyses on these data include de novo transcriptome assembly and differential expression analyses. Sample-specific and combined transcriptomes, gene annotations, and differential expression results are described in this data note and are available from publicly-available databases.
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