The Blood Lead Level and Fresh Blood Quick Native Immune Reaction

Abstract

Although we have gained much information about lead-induced organ damage, the effect of blood lead level on fresh blood quick native immune reaction on cancer cells is yet to be determined. The aim of study is evaluating the significance of blood level, as an indicator for environmental lead, and fresh blood quick native immune reaction on cancer cells as an effect indicator, to determinate the correlation between the content of blood lead and quick native immune reaction on cancer. The 120 children from area to exposure environmental lead were recruited into the study using cancer cells adding in fresh anti-coagulate blood to incubation at 37°C for 30 minutes and study of blood lead level using graphite stove atom absorption spectrophotometer, with blood lead level of 0.48μmol/L as a cut off value. blood lead level and fresh blood quick native immune reaction on cancer cells expression were measured with graphite furnace atomic absorption spectroscopy and using cancer cells adding in fresh anti-coagulate blood to incubation at 37°C for 30 minutes respectively. Student t test was used in data analysis, and correlation analysis was used to assess the correlation between blood lead level and expression of cancer cells adding in fresh anti-coagulate. Date from all 120 children was used for data analysis. Expression of TRR and TLR were lower in Group 1(lead level ≥0.48μmol/L) than Group 3(lead level 0.24μmol/L)(t=3.48, 2.32,P<0.01). When the blood lead level was ≥0.48μmol/L, the blood lead level showed significant inverse correlation with TRR and TLR(r=-0.703,-0.606 P<0.01). The result revealed the level of fresh blood quick native immune reaction on cancer cells was a significant difference between groups of high and low blood lead levels. There was correlation in the level of blood lead and fresh blood quick native immune reaction on cancer cells. The result suggested that the high blood lead level may be regarded as an adverse effect on children’s immune function especially on TRR.TLR percentage when exposed environmental lead.