Abstract
Distant hybridization often results in the generation of naturally gynogenic offspring. However, there are currently few reports about fertile natural gynogenetic bream. In this study, we established a natural gynogenetic blunt snout bream (NGBSB-YT, F1) through distant hybridization from female blunt snout bream (Megalobrama amblycephala, BSB) × male Bleeker's yellow tail (Xenocypris davidi Bleeker, YT), and further obtained NGBSB1-YT (F2) by self-cross. Except for the morphological characteristics, the DNA content, chromosome numbers, and 5 S rDNA sequences of NGBSB1-YT had no significant difference compared to those of BSB. The results of gonad sections showed that NGBSB1-YT was bisexual. The NGBSB2-YT (F3) obtained by self-cross further proved the fertility of NGBSB1-YT. Interestingly, the results of microsatellite (prime XD007) analysis and sox9 sequence analysis confirmed that paternal DNA fragments were inserted in NGBSB1-YT. This study has established a lineage of natural gynogenetic bream(F1-F3), that provides a reference for improvement of the theory of natural gynogenesis in fish. The NGBSB-YT lineage can be used as a new type of germplasm resource of bream.
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